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A Study of Formation and Fragmentation of Ionic Complexes of α‐Amino Acids and Peptides with Al(III)–Glycerol by Fast Atom Bombardment Mass Spectrometry
Author(s) -
Saraswathi Mandapati,
Miller Jack M.
Publication year - 1996
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(199610)10:13<1706::aid-rcm723>3.0.co;2-p
Subject(s) - chemistry , adduct , glycerol , fast atom bombardment , trifluoroacetic acid , amino acid , fragmentation (computing) , mass spectrometry , metal ions in aqueous solution , dissociation (chemistry) , ion , amide , medicinal chemistry , organic chemistry , chromatography , biochemistry , computer science , operating system
Complexation of aluminum ions {Al(III) in glycerol} with α‐amino acids has been studied by FAB mass spectrometry. Twelve α‐amino acids and cysteine methyl ester interact with different Al(III)‐containing ions and form several cluster ions such as [M+117] + , [117={A1(III)+glycerol−2H}]; [M+231] + , [231={Al(III)+glycerol+ trifluoroacetic acid−2H}]; [M+233] + , [233={2Al(III)+2glycerol−5H}]; [2M+Al−2H] + and [2M+117] + . Fragmentation of these adduct ions led to the formation of mainly metal‐containing product ions. Fragmentation pathways were demonstrated with the help of the dissociation of Leu‐d 3 , His‐d 4 , Tyr‐d 4 , Ser‐d 4 , Cys‐d 4 and Cys methyl ester‐d 3 ion complexes in glycerol‐d 3 . Aliphatic, aromatic and α‐amino acids possessing functional side chains show differences in the fragmentation of [M+117] + ions. Fourteen peptides of different chain lengths (two through six amino acids) also form adducts with Al(III)‐glycerol. Dissociation of these complex ions, especially [M+Al−2H] + ions provides information about side‐chain functional groups and some sequence information. The site of the metal‐ion interaction is mostly at the amide nitrogens.