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UV Optical Absorption of Matrices Used for Matrix‐assisted Laser Desorption/Ionization
Author(s) -
Allwood D. A.,
Dreyfus R. W.,
Perera I. K.,
Dyer P. E.
Publication year - 1996
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(199610)10:13<1575::aid-rcm658>3.0.co;2-c
Subject(s) - chemistry , desorption , molar absorptivity , analytical chemistry (journal) , absorption (acoustics) , matrix (chemical analysis) , matrix assisted laser desorption electrospray ionization , crystallite , ionization , matrix assisted laser desorption/ionization , chromatography , adsorption , organic chemistry , optics , crystallography , ion , physics
Previous modelling of the matrix‐assisted laser desorption/ionization (MALDI) process has had to rely on matrix solution optical absorptivities whereas the value for the solid phase is more appropriate. Absorption bands are usually broader for solids than for solutions due to the higher concentration in the former, hence solution values generally overestimate the solid absorptivity. In order to rectify this shortcoming, the present work reports values of the optical absorption coefficient (α) of sublimed MALDI matrix films with measured thicknesses of 50 to 600 nm. The matrix compounds studied were ferulic acid, sinapinic acid, α‐cyano‐4‐hydroxycinnamic acid, 2,5‐dihydroxybenzoic acid, (2,5‐DHB), nicotinic acid and coumarin 120. In general the films showed integrated cross‐sections (oscillator strengths) similar to those determined by measurements on the respective solutions. Scanning electron microscopy of 2,5‐DHB and coumarin 120 films showed them to consist of a uniform layer of sub‐micron (⪅300 nm) crystallites.