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Step‐by‐step Control by Time‐of‐flight Secondary Ion Mass Spectrometry of a Peptide Synthesis Carried Out on Polymer Beads
Author(s) -
Drouot C.,
Enjalbal C.,
Fulcrand P.,
Martinez J.,
Aubagnac J. L.,
Combarieu R.,
de Puydt Y.
Publication year - 1996
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(199609)10:12<1509::aid-rcm663>3.0.co;2-g
Subject(s) - chemistry , mass spectrometry , secondary ion mass spectrometry , fragmentation (computing) , ion , peptide , time of flight , chromatography , combinatorial chemistry , cleavage (geology) , top down proteomics , polymer , analytical chemistry (journal) , tandem mass spectrometry , protein mass spectrometry , organic chemistry , biochemistry , fracture (geology) , computer science , engineering , geotechnical engineering , operating system
The use of time‐of‐flight secondary ion mass spectrometry (Tof‐SIMS) for the step‐by‐step control of a peptide synthesis on a solid support is illustrated. The positive secondary ions, issuing from energetic fragmentation, are characteristic of the terminal groups of the step under investigation. The negative secondary ions result from the cleavage of the benzyl ester link between the polymer support and the first amino acid, and provide direct identification of the growing sequence. Tof‐SIMS offers this unique analytical possibility without any preliminary separation of the peptide from its support.