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Investigation of Newly Synthesized Endothelin Peptides by High‐performance Liquid Chromatography Coupled to Electrospray Mass Spectrometry
Author(s) -
Rovatti Luca,
Curcuruto Ornella,
Hamdan Mahmoud,
Cassano Ersilia,
Galoppini Claudia,
Rovero Paolo
Publication year - 1996
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(199609)10:12<1504::aid-rcm640>3.0.co;2-5
Subject(s) - chemistry , chromatography , electrospray , mass spectrometry , peptide , electrospray mass spectrometry , high performance liquid chromatography , protein mass spectrometry , electrospray ionization , biochemistry
A number of newly synthesized endothelin peptides have been examined by high performance liquid chromatography/electrospray mass spectrometry (HPLC/ES‐MS). The presented data allowed reliable identification of both the target peptides and a number of associated side products including the target peptide in methylated form and peptide sequences with relative molecular masses lower than those desired. Characteristic fragments in the ES mass spectra permitted differentiation between the methylation sites involving the carboxylic functions of the C‐terminus and the side‐chain of the aspartic acid.