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A Three‐point Calibration Procedure for Matrix‐assisted Laser Desorption/Ionization Mass Spectrometry Utilizing Multiply Charged Ions and Their Mean Initial Velocities
Author(s) -
Costa Vera César,
Zubarev Roman,
Ehring Hanno,
Hakansson Per,
Sunqvist Bo. U. R.
Publication year - 1996
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(199609)10:12<1429::aid-rcm600>3.0.co;2-w
Subject(s) - chemistry , mass spectrometry , calibration , ion , ionization , analytical chemistry (journal) , matrix (chemical analysis) , polyatomic ion , time of flight mass spectrometry , laser , mass spectrum , desorption , matrix assisted laser desorption electrospray ionization , atomic physics , chromatography , thermal ionization mass spectrometry , optics , physics , organic chemistry , quantum mechanics , adsorption
This paper reports the results obtained by using a new calibration procedure for linear time‐of‐flight matrix‐assisted laser desorption/ionization mass spectrometry (MALDI). The procedure takes into account the usual MALDI spectra structure by considering the multiply charged molecular ions and the mean initial velocities as a self calibrating property. The mean velocities for all molecular ions are taken to be the same and the procedure is based on the solution of a number of equations (at least three) which relate time‐of‐flight to mass. Three times better accuracy was obtained in comparison with the normal internal calibration procedure.