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Accurate Monoisotopic Mass Measurements of Peptides: Possibilities and Limitations of High Resolution Time‐of‐flight Particle Desorption Mass Spectrometry
Author(s) -
Zubarev Roman A.,
Håkansson Per,
Sundqvist Bo
Publication year - 1996
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(199608)10:11<1386::aid-rcm652>3.0.co;2-t
Subject(s) - monoisotopic mass , chemistry , mass spectrometry , time of flight , time of flight mass spectrometry , analytical chemistry (journal) , ion , resolution (logic) , chromatography , ionization , organic chemistry , artificial intelligence , computer science
The possibilities and limitations of time‐of‐flight particle‐desorption mass spectrometry are analysed from the standpoint of accuracy of monoisotopic mass determination. For an instrument with mass reflector, typical mass accuracy was found to be ca . 20 ppm (standard deviation). The main limitation was found to be the mechanism of secondary ion production itself. A fraction of biological ions are formed in the gas phase and therefore exhibit both time delay and energy deficit. This leads to a limited resolving power and non‐symmetric ion peaks. A peak shape model, taking into account gas‐phase ion formation, is built and analysed, and a simple correction procedure proposed. The application of the correction has increased the mass accuracy to 12 ppm (standard deviation). It is shown that further progress cannot be achieved by a correction procedure without instrumental improvements.