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Detection and Characterization of Fumonisin Mycotoxins by Liquid Chromatography/Electrospray Ionization Using Ion Trap and Triple Quadrupole Mass Spectrometry
Author(s) -
Josephs Jonathan L.
Publication year - 1996
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(199608)10:11<1333::aid-rcm620>3.0.co;2-f
Subject(s) - chemistry , triple quadrupole mass spectrometer , ion trap , mass spectrometry , chromatography , electrospray ionization , quadrupole ion trap , electrospray , tandem mass spectrometry , selected reaction monitoring , fumonisin b1 , analytical chemistry (journal) , top down proteomics , mycotoxin , food science
Electrospray ionization (ESI) high‐performance liquid chromatography/mass spectrometry using both ion trap and triple quadrupole mass spectrometers has been utilized for the detection and characterization of fumonisin mycotoxin impurities in a purified sample of fumonisin B 1 (FB 1 ). Multi‐stage tandem mass spectrometry (MS n ) and liquid chromatography/tandem mass spectrometry (LC/MS/MS) of FB 1 and the fifteen synthetically prepared methyl esters of FB 1 on the ion trap instrument allowed investigation of the numerous fragmentation pathways available for this compound class. Data‐dependent LC/MS/MS precursor‐ion scan to product‐ion scan experiments, based on the ion trap MS n experiments, were carried out on a triple quadrupole instrument and facilitated the detection of twelve, and the structural characterization of eight, minor impurities in the purified sample of FB 1 . Data‐dependent LC/MS/MS full scan to product‐ion scan experiments on the ion trap instrument resulted in the detection and characterization of fifteen further impurities in the purified sample of FB 1 ; this number included the four uncharacterized impurities detected in the triple quadrupole LC/MS/MS experiments.