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A Study of the Enzymatic Oligomerization of 5,7‐Dihydroxytryptamine using Matrix‐assisted Laser Desorption/Ionization Mass Spectrometry
Author(s) -
Bertazzo Antonella,
Costa Carlo,
Allegri Graziella,
Favretto Donata,
Traldi Pietro
Publication year - 1996
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19960731)10:10<1299::aid-rcm588>3.0.co;2-x
Subject(s) - chemistry , mass spectrometry , desorption , ionization , matrix (chemical analysis) , matrix assisted laser desorption/ionization , analytical chemistry (journal) , chromatography , organic chemistry , adsorption , ion
The reaction between 5,7‐dihydroxytryptamine (5,7‐DHT) and mushroom tyrosinase was studied by matrix‐assisted laser desorption/ionization mass spectrometry of various samples drawn after different reaction times (0, 30, 60, 120, 240 and 360 min), ultrafiltered and immediately lyophilized. In order to evaluate the role of non‐enzymatic oxidation, a portion of the ultrafiltered sample was left to react under an oxygen stream for a further 24 hr, lyophilized and analysed by the same method. Results show that, in the case of 5,7‐DHT, oligomerization products are obtained which are structurally different from those produced by the same enzymatic reaction on 5,6‐DHT. This is explained by the tendency of 5,7‐DHT towards oxidation, since the oligomerization products originate from oxidized forms of 5,7‐DHT.