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Simple Analysis of Plasmalogens in Erythrocytes Using Gas Chromatography/Mass Spectrometry with Selected‐ion Monitoring Acquisition
Author(s) -
Caruso U.
Publication year - 1996
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19960731)10:10<1283::aid-rcm298>3.0.co;2-n
Subject(s) - plasmalogen , chemistry , chromatography , peroxisome , mass spectrometry , peroxisomal disorder , biochemistry , membrane , phospholipid , gene
Plasmalogens are a unique class of ether‐phospholipids whose role, even though not fully defined, is essential. Their biosynthesis starts in peroxisomes, therefore, plasmalogen analysis is fundamental in the study of peroxisomal disorders. The present work reports a simple method for plasmalogen determination in erythrocytes for use in the study of peroxisomal disorders in humans; the procedure is based on two other methods that have previously been reported and employs GC/MS for separation and detection.