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The in vivo Glyco‐oxidation of α‐ and β‐Globins Investigated by Matrix‐assisted Laser Desorption/Ionization Mass Spectrometry
Author(s) -
Lapolla A.,
Fedele D.,
Aronica R.,
Garbeglio M.,
D'Alpaos M.,
Seraglia R.,
Traldi P.
Publication year - 1996
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19960715)10:9<1133::aid-rcm581>3.0.co;2-c
Subject(s) - chemistry , glycation , mass spectrometry , surface enhanced laser desorption/ionization , matrix assisted laser desorption/ionization , globin , desorption , chromatography , ionization , matrix (chemical analysis) , analytical chemistry (journal) , sample preparation in mass spectrometry , hemoglobin , biochemistry , organic chemistry , adsorption , ion , electrospray ionization , receptor
Matrix‐assisted laser desorption/ionization (MALDI) has been employed for the determination of molecular weights of α‐ and β‐globins obtained from blood samples of healthy and diabetic subjects. Glycated species, originating from the reaction of glucose with the proteins, are easily evident. The MALDI measurements allowed one to distinguish between simple glycation and glyco‐oxidation processes of haemoglobin. In fact, together with the products arising from simple glucose condensation on the two proteins, compounds at different oxidation levels were evident. Due to its high specificity, MALDI must be considered a valuable tool for diagnosis in the diabetes field.