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Investigation of Newly Synthesized Peptides by Capillary Zone Electrophoresis/Electrospray Mass Spectrometry
Author(s) -
Hamdan Mahmoud,
Curcuruto Ornella,
Vigano' Stefania,
Rovero Paolo
Publication year - 1996
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19960715)10:9<1128::aid-rcm589>3.0.co;2-b
Subject(s) - chemistry , chromatography , mass spectrometry , electrospray , capillary electrophoresis , capillary electrophoresis–mass spectrometry , electrospray mass spectrometry , protein mass spectrometry , electrospray ionization
A series of newly synthesized peptides ( M r =1600–2250 Da), corresponding to portions of the extracellular domain of human granulocyte–macrophage colony stimulating factor receptor α subunit have been examined by capillary zone electrophoresis/electrospray‐mass spectrometry (CZE/ES‐MS). The separation efficiency of CZE combined with the specificity of mass spectrometry allowed rapid and reliable identification of the target peptides and a number of associated side products. In solid‐phase synthesis of peptides and small proteins such side products are inevitable, therefore the use of CZE and/or high‐performance liquid chromatography combined with mass spectrometry is gaining a central role in such synthesis procedures.