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Electrospray Mass Spectrometry for Detection and Characterization of Purified Cricket Paralysis Virus (CrPV)
Author(s) -
Despeyroux D.,
Phillpotts R.,
Watts P.
Publication year - 1996
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19960610)10:8<937::aid-rcm598>3.0.co;2-9
Subject(s) - capsid , chemistry , picornavirus , mass spectrometry , electrospray , chromatography , virus , molecular mass , virology , biochemistry , enzyme , biology , genome , gene
Electrospray mass spectrometry has been used to detect and characterize the capsid proteins of a non‐disrupted sample of cricket paralysis virus. By direct injection and by on‐line liquid chromatography/mass spectrometry of the intact virus, three capsid proteins (VP1, VP2 and VP3) were detected and their relative molecular masses (RMM) accurately determined. In addition to these proteins, a fourth polypeptide (RMM=6012 Da) was detected which, by analogy with mammalian picornaviruses, may correspond to the capsid protein VP4. The finding of this latter protein now reduces the uncertainty of the classification of CrPV as a picornavirus.