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High Sensitivity Collisionally‐activated Decomposition Tandem Mass Spectrometry on a Novel Quadrupole/Orthogonal‐acceleration Time‐of‐flight Mass Spectrometer
Author(s) -
Morris Howard R.,
Paxton Thanai,
Dell Anne,
Langhorne Jean,
Berg Matthias,
Bordoli Robert S.,
Hoyes John,
Bateman Robert H.
Publication year - 1996
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19960610)10:8<889::aid-rcm615>3.0.co;2-f
Subject(s) - chemistry , mass spectrometry , tandem mass spectrometry , tandem , electrospray , hybrid mass spectrometer , triple quadrupole mass spectrometer , top down proteomics , analytical chemistry (journal) , mass spectrum , selected reaction monitoring , quadrupole , protein mass spectrometry , time of flight mass spectrometry , spectrometer , chromatography , ion , atomic physics , ionization , optics , physics , aerospace engineering , engineering , organic chemistry
Consideration of the special problems encountered in ultra‐high sensitivity biopolymer sequencing studies has led to the development of a novel quadrupole/orthogonal‐acceleration time‐of‐flight tandem mass spectrometer described for the first time here. The performance characteristics of this new geometry are demonstrated, including fully resolved daughter‐ion spectra with mass accuracies of 0.1 dalton, which allow removal of interpretation ambiguities and easy differentiation of charge states even in weak collisionally‐activated decomposition tandem mass spectra. The instrument has been applied to a variety of biopolymer research problems, including the structure determination of major histocompatibility complex peptide antigens using liquid chromatography/electrospray mass spectrometry and nanoflow‐electrospray tandem mass spectrometry, and sequencing capability in the low‐femtomole and attomole ranges is demonstrated.