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Photochemical Versus Thermal Mechanisms in Matrix‐assisted Laser Desorption/Ionization Probed by Back Side Desorption
Author(s) -
Ehring Hanno,
Costa Cesar,
Demirev Plamen A.,
Sundqvist Bo U. R.
Publication year - 1996
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(199605)10:7<821::aid-rcm566>3.0.co;2-a
Subject(s) - chemistry , soft laser desorption , photoexcitation , desorption , photochemistry , matrix assisted laser desorption electrospray ionization , laser , thermal desorption , ionization , excited state , ion , atmospheric pressure laser ionization , analytical chemistry (journal) , mass spectrometry , photoionization , matrix assisted laser desorption/ionization , atomic physics , adsorption , optics , organic chemistry , chromatography , physics
The contribution of photochemical and thermal effects in the formation and ejection of biomolecular ions in matrix‐assisted laser desorption/ionization; (MALDI) is studied by comparing desorption from one‐and‐the‐same sample in two incident laser geometries—irradiating the sample front, or irradiating the back side through a 2000 Å gold layer. Such a set‐up results in the efficient decoupling of photoexcitation from thermal processes. Our results indicate that laser‐induced photochemistry, e.g. charge transfer reactions between electronically‐excited matrix molecules and the analyte, plays a major role in the MALDI process.