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Matrix‐assisted Laser Desorption/Ionization Using a New Tandem Quadrupole Ion Storage Trap, Time‐of‐flight Mass Spectrometer
Author(s) -
Kofel Peter,
Stöckli Markus,
Krause Jörn,
Schlunegger Urs P.
Publication year - 1996
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(199604)10:6<658::aid-rcm517>3.0.co;2-z
Subject(s) - chemistry , ion trap , mass spectrometry , ion trapping , ion , time of flight mass spectrometry , quadrupole , analytical chemistry (journal) , ionization , quadrupole mass analyzer , ion source , atomic physics , time of flight , ambient ionization , soft laser desorption , tandem , penning trap , desorption , chemical ionization , matrix assisted laser desorption/ionization , chromatography , physics , materials science , organic chemistry , adsorption , composite material
A tandem quadrupole ion‐storage trap, time‐of‐flight instrument using matrix‐assisted laser desorption/ionization has been constructed. Desorbed ions are first trapped in an RF field and collisionally relaxed prior to time‐of‐flight mass analysis. So far masses up to 90 kDa, a resolution of up to 700 and trapping times of up to 10 minutes have been measured with RF frequencies and amplitudes as low as 20 kHz and 40 V, respectively. The effects of pulse synchronization with the RF voltage and of extraction voltage variation on instrument performance have been investigated. Molecular ions of melittin have been found to undergo reactions in the gas phase during storage.