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Study of Frozen Solutions of Nucleic Acid Nitrogen Bases by Means of Low Temperature Fast‐atom Bombardment Mass Spectrometry
Author(s) -
Boryak Oleg A.,
Kosevich Marina V.,
Shelkovsky Vadim S.,
Blagoy Yuriy P.
Publication year - 1996
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19960131)10:2<197::aid-rcm457>3.0.co;2-g
Subject(s) - chemistry , glycerol , mass spectrometry , fast atom bombardment , nucleic acid , nitrogen , hydrate , mass spectrum , ion , liquid nitrogen , inorganic chemistry , analytical chemistry (journal) , chromatography , organic chemistry , biochemistry
Frozen water and glycerol solutions of pyrimidine nitrogen bases of nucleic acids were studied by means of low temperature fast‐atom bombardment mass spectrometry. The reflection of some phenomena of cryobiological interest was observed in the peculiarities of the low temperature fast‐atom bombardment mass spectral patterns. The anomalously high abundance of clusters of nitrogen bases with alkali‐metal ions in the spectra of frozen water solutions in contrast to complete absence of such clusters for glycerol solutions reflects the concentrating of the solute during freezing and its prevention by the cryoprotector, glycerol. The effect of dehydration during freezing is reflected in the absence of hydrate clusters, while bases solvated by glycerol are recorded.