Premium
An Electrospray Investigation on in vitro Glycation of Ribonuclease
Author(s) -
Lapolla Annunziata,
Fedele Domenico,
Baldo Lorenzo,
Aronica Rosaria,
Raverdino Vittorio,
Catinella Silvia,
Seraglia Roberta,
Traldi Pietro
Publication year - 1996
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19960131)10:2<178::aid-rcm454>3.0.co;2-o
Subject(s) - chemistry , electrospray , chromatography , glycation , electrospray mass spectrometry , rnase p , mass spectrometry , ribonuclease , electrospray ionization , substrate (aquarium) , biochemistry , rna , oceanography , receptor , gene , geology
The in vitro reactions of RNase with different concentrations of glucose or fructose have been studied by means of electrospray mass spectrometry coupled with microcolumn high performance liquid chromatography. The results obtained have shown that, subsequent to the protein glycation, a series of cross‐linking products are generated. Their molecular weights demonstrate that severe degradation processes of the proteic substrate takes place after the cross‐linking process.