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High‐performance Liquid Chromatography/Tandem Mass Spectrometry Identification of Salmon Calcitonin Degradation Products in Aqueous Solution Preparations
Author(s) -
Silvestro Luigi,
Savu Simona Rizea
Publication year - 1996
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19960131)10:2<151::aid-rcm434>3.0.co;2-c
Subject(s) - chemistry , chromatography , high performance liquid chromatography , mass spectrometry , aqueous solution , tandem mass spectrometry , liquid chromatography–mass spectrometry , salmon calcitonin , calcitonin , organic chemistry , medicine
Degradation impurities have been investigated, by means of high‐performance liquid chromatography (HPLC)/mass spectrometry, in commercial ampoules of salmon calcitonin, aqueous solution, stored for up to 2 years. Oxidation derivatives of the cysteine residues in positions 1 and 7, with cleavage of the disulfide bridge, were observed in all samples. Using our reversed‐phase separation, these oxidation derivatives coeluted and presented an HPLC behavior identical to the derivative of salmon calcitonin with the disulfide bridge reduced. In a previous study this last compound was tentatively identified, without confirmation by mass spectrometry, as a major degradation product of salmon calcitonin in aqueous solution.