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Coaxial Continuous‐flow Fast‐atom Bombardment vs Electrospray Ionization: a Sensitivity Comparison on a Magnetic Sector Instrument
Author(s) -
Deterding Leesa J.,
Khaledi Morteza,
Tomer Kenneth B.
Publication year - 1996
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19960115)10:1<60::aid-rcm446>3.0.co;2-3
Subject(s) - chemistry , electrospray ionization , coaxial , electrospray , ionization , mass spectrometry , analytical chemistry (journal) , continuous flow , chromatography , ion , physics , organic chemistry , electrical engineering , engineering , mechanics
The performance of an electrospray (ESI) source was evaluated and compared to that of a coaxial continuous‐flow fast‐atom bombardment source on a magnetic mass spectrometer using ten different peptides over the mass range of 400 to 3500 Da and using a variety of scanning modes. Results show that sensitivities using the two ionization techniques are similar, with limits‐of‐detection in the attomole to low femtomole range. In addition, proteins can be routinely detected using ESI at femtomole levels. The observance of the noncovalent complex of RNase A and cytidine 2′‐monophosphate yields evidence that these complexes can be studied using instruments that operate at high accelerating voltages.