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Isolation and Characterization of Conopeptides by High‐performance Liquid Chromatography Combined with Mass Spectrometry and Tandem Mass Spectrometry
Author(s) -
Jones Alun,
Bingham JonPaul,
Gehrmann John,
Bond Trudy,
Loughnan Marion,
Atkins Ann,
Lewis Richard J.,
Alewood Paul F
Publication year - 1996
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19960115)10:1<138::aid-rcm442>3.0.co;2-3
Subject(s) - chemistry , chromatography , mass spectrometry , tandem mass spectrometry , liquid chromatography–mass spectrometry , tandem , protein mass spectrometry , materials science , composite material
The sensitivity of mass spectrometry combined with the separatory power of high‐performance liquid chromatography was used to investigate the venom of individual cone shells. This analysis has revealed that cone venoms contain a complex mixture of peptides which vary quantitatively and qualitatively both between and within species. A differential alkylation procedure followed by tandem mass spectrometric analysis can be used to determine the disulfide bond connectivity in these small, cysteine‐rich peptides.