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A comparison of different density gradients and antibodies for enrichment of fetal erythroblasts by MACS
Author(s) -
Troeger Carolyn,
Holzgreve Wolfgang,
Hahn Sinuhe
Publication year - 1999
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/(sici)1097-0223(199906)19:6<521::aid-pd578>3.0.co;2-n
Subject(s) - fetus , andrology , antibody , antigen , cord blood , biology , medicine , immunology , pregnancy , genetics
The enrichment of fetal cells, in particular fetal erythroblasts from the blood of pregnant women offers a promising non‐invasive alternative for prenatal diagnosis. The purpose of this study was to compare the retrieval of erythroblasts by different density gradients and different antibodies against erythroid surface antigens, in both a model test system and in blood samples of pregnant women. We enriched erythroblasts from artificial mixtures of cord and adult blood (1:50) and from 16 ml of peripheral blood from pregnant women at a mean gestational age of 14+2 weeks. The yield of erythroblasts was calculated and compared using Wilcoxon's matched‐pairs signed‐rank test. In the artificial mixture most erythroblasts were retrieved using the heaviest density gradient (specific density of 1119) followed by antibody‐labelled magnetic cell sorting (MACS). With anti‐GPA the yield of erythroblasts from the artificial mixture was highest (9362.5 erythroblasts) compared with anti‐CD36 (5164.3), anti‐i (3455.2), anti‐CD71 (3055.8) and HAE9 (2364.2). The difference between anti‐GPA and anti‐CD71, HAE9 and anti‐i was significant ( p =0.0277). The enrichment of erythroblasts from peripheral blood of pregnant women showed similar results. The yield of erythroblasts using anti‐GPA was the highest. These results enable us to simplify our enrichment protocols to a single density gradient of 1119 specific density followed by MACS, with anti‐GPA. Copyright © 1999 John Wiley & Sons, Ltd.