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Prenatal genotyping of the Duffy blood group system by allele‐specific polymerase chain reaction
Author(s) -
Hessner Martin J.,
Pircon Richard A.,
Johnson Susan T.,
Luhm Robert A.
Publication year - 1999
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/(sici)1097-0223(199901)19:1<41::aid-pd463>3.0.co;2-a
Subject(s) - genotyping , concordance , polymerase chain reaction , amniocentesis , allele , prenatal diagnosis , amniotic fluid , biology , fetus , genotype , immunology , genetics , pregnancy , gene
Maternal allo‐immunization to antigens of the Duffy blood group system can result in haemolytic disease of the newborn (HDN), therefore, the application of allele‐specific polymerase chain reaction (ASPCR) for prenatal genotyping of the Duffy antigen system to identify pregnancies at risk for HDN was evaluated. Oligonucleotide primers were designed for ASPCR of FYA , FYB and nullFY alleles. A validation study was performed using DNA isolated from 94 serotyped whole blood samples and 8 amniocentesis samples. A concordance rate of 100 per cent was observed between serotyping and ASPCR detection of the FYA, FYB and nullFY alleles. This assay is particularly useful for rapid genotyping of fetal amniotic cells to identify pregnancies at risk for HDN due to maternal–fetal incompatibilities within the Duffy blood group system. Copyright © 1999 John Wiley & Sons, Ltd.