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Multiple genetic diagnoses from single cells using multiplex PCR: reliability and allele dropout
Author(s) -
Findlay Ian,
Matthews Paul,
Quirke Phil
Publication year - 1998
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/(sici)1097-0223(199812)18:13<1413::aid-pd496>3.0.co;2-1
Subject(s) - allele , multiplex , medical diagnosis , multiplex polymerase chain reaction , reliability (semiconductor) , genetics , biology , medicine , polymerase chain reaction , gene , pathology , power (physics) , physics , quantum mechanics
We used a multiplex fluorescent PCR system containing seven primer sets on single cells from three different cell types (buccal, corneal and blastomere cells) and more than 3500 heterozygous alleles to investigate reliability and extent of allele dropout in multiplex PCRs at the single cell level. All three cell types gave similarly high reliability, accuracy and allele dropout rates, with similar reliability between singleplex and multiplex PCRs. Allele dropout was also consistent between the three cell types and did not significantly increase as allele size increased. These results indicate that multiplex fluorescent PCR is a reliable and accurate method of obtaining multiple diagnosis (eight chromosomes simultaneously) from single cells and maximizes the information available from single cell analysis. Copyright © 1998 John Wiley &Sons, Ltd.