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Prenatal genotyping of Jk a and Jk b of the human Kidd blood group system by allele‐specific polymerase chain reaction
Author(s) -
Hessner Martin J.,
Pircon Richard A.,
Johnson Susan T.,
Luhm Robert a.
Publication year - 1998
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/(sici)1097-0223(199812)18:12<1225::aid-pd434>3.0.co;2-d
Subject(s) - genotyping , concordance , polymerase chain reaction , amniocentesis , amniotic fluid , allele , prenatal diagnosis , biology , genotype , microbiology and biotechnology , genetics , medicine , fetus , pregnancy , gene
Abstract An allele‐specific polymerase chain reaction (ASPCR) assay for prenatal genotyping of the Kidd antigen system in order to identify pregnancies at risk for haemolytic disease of the newborn (HDN) was developed. Oligonucleotide primers were designed for ASPCR of JKA and JKB . A validation study was performed using DNA isolated from 54 serotyped whole blood samples and 8 amniocentesis samples. A concordance rate of 100 per cent was observed between serotyping and ASPCR detection of the JKA and JKB alleles. Experiments were conducted to quantify the maternal contamination that could be tolerated in Kidd ASPCR assays. The sensitivity of this assay ranged from 0·2 per cent when detecting the presence of JKB and JKA background, to 2 per cent for detecting the presence of JKA in a JKB background. This sensitive assay is particularly useful for rapid genotyping of fetal amniotic cells to identify pregnancies at risk for HDN due to incompatibilities within the Kidd blood group system. Copyright © 1998 John Wiley & Sons, Ltd.