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Quantitative evaluation of fluorescence in situ hybridization (FISH) signals in uncultured coelomic cells
Author(s) -
Crüger D. G.,
BruunPetersen G.,
Kølvraa S.
Publication year - 1997
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/(sici)1097-0223(199707)17:7<635::aid-pd127>3.0.co;2-2
Subject(s) - in situ hybridization , hybridization probe , fluorescence in situ hybridization , in situ , microbiology and biotechnology , satellite dna , biology , molecular probe , dna , dna–dna hybridization , nucleic acid thermodynamics , ctd , satellite , fish <actinopterygii> , chemistry , chromosome , base sequence , gene , physics , genetics , gene expression , geology , fishery , oceanography , organic chemistry , astronomy
Abstract Uncultured coelomic cells were hybridized with alpha satellite DNA probes representing chromosomes X, Y, 18, and 13/21 in order to evaluate the distribution of hybridization signals obtained by fluorescence in situ hybridization (FISH) analysis of this cell type. Cells from 26 samples were hybridized with the X probe and the Y probe was hybridized with cells from 25 of the samples. Cells from 16 and 11 samples were hybridized with an 18 alpha satellite DNA probe and 13/21 alpha satellite probe, respectively. The evaluation demonstrated that FISH with X, Y, 18, and 13/21 alpha satellite DNA probes on uncultured coelomic cells is a reliable technique since the distribution of hybridization signals is comparable to that seen in uncultured amniotic fluid cells. © 1997 John Wiley & Sons, Ltd.