Premium
Monoclonal antibody 57B stains tumor tissues that express gene MAGE‐A4
Author(s) -
Landry Claire,
Brasseur Francis,
Spagnoli Giulio C.,
Marbaix Etienne,
Boon Thierry,
Coulie Pierre,
Godelaine Danièle
Publication year - 2000
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(20000615)86:6<835::aid-ijc12>3.0.co;2-n
Subject(s) - monoclonal antibody , transfection , microbiology and biotechnology , stain , recombinant dna , antibody , gene , biology , immunohistochemistry , monoclonal , staining , pathology , medicine , immunology , genetics
Monoclonal antibody (Mab) 57B, which was raised against a recombinant MAGE‐A3 protein, was tested for its ability to stain cells expressing various members of the MAGE‐A gene family. COS‐7 cells transfected with cDNAs encoding MAGE‐A1, A2, A3, A4, A6 , or A12 were stained, whereas those transfected with MAGE‐A8, A9, A10 , or A11 cDNAs were not. However, in tissue sections, we observed a different pattern of staining: the antibody effectively stained the tumors that expressed MAGE‐A4 and only these tumors, regardless of the expression of the other MAGE‐A genes. It seems, therefore, that at the level of MAGE gene expression found in tumors, a level clearly lower than that observed in transfected COS cells, only the MAGE‐A4 protein can be reliably detected. We conclude that the 57B Mab should be useful for tumor diagnosis related to therapeutic vaccination involving MAGE‐A4. Int. J. Cancer 86:835–841, 2000. © 2000 Wiley‐Liss, Inc.