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Expression of p34 cdc2 and cyclins A and B compared to other proliferative features of non‐Hodgkin's lymphomas: A multivariate cluster analysis
Author(s) -
Leoncini Lorenzo,
Cossu Antonio,
Megha Tiziana,
Bellan Cristiana,
Lazzi Stefano,
Luzi Pietro,
Tosi Piero,
Barbini Paolo,
Cevenini Gabriele,
Pileri Stefano,
Giordano Antonio,
Kraft Rainer,
Laissue Jean A.,
Cottier Hans
Publication year - 1999
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19991008)83:2<203::aid-ijc10>3.0.co;2-0
Subject(s) - biology , mitotic index , cyclin , cell cycle , cyclin b , lymphoma , mitosis , cyclin a , cancer research , cyclin dependent kinase 1 , cell growth , cell , immunology , microbiology and biotechnology , genetics
In view of recent knowledge on proteins regulating the cell cycle, we re‐evaluated proliferative features of 98 diffusely growing non‐Hodgkin's lymphomas. The combined use of 5 proliferation–associated variables (mitotic indices and percentages of Ki‐67 + , p34 cdc2+ , cyclin A + and cyclin B + cells) and their entry into a multivariate cluster analysis separated, without overlaps, the entire cohort into 3 groups (clusters) with (1) low, (2) intermediate and (3) high proliferative activity. Conversely, bivariate plots exposed considerable cluster overlaps. Multivariate stepwise discriminant analysis of all cases revealed a decreasing order of discriminant power for % Ki‐67 + cells > % p34 cdc2+ cells > mitotic index > % cyclin A + cells > % cyclin B + cells. The combined use of 2 variables only, mitotic index and % p34 cdc2+ cells, allowed a clear‐cut separation of clusters 2 and 3. In bivariate plots, correlations were best between % Ki‐67 + cells and % cyclin A + cells and between mitotic indices and % cyclin B + cells. Except for chronic lymphocytic leukemias, immunocytomas and marginal zone lymphomas (all in cluster 1), individual lymphoma entities were distributed among at least 2 clusters. There was, however, a marked preponderance of mantle cell lymphomas and diffuse follicular center lymphomas in cluster 1 and of diffuse large B‐cell lymphomas and peripheral T‐cell lymphomas in cluster 2. Anaplastic large‐cell lymphomas predominated in cluster 3 and responded best to therapy. Int. J. Cancer 83:203–209, 1999. © 1999 Wiley‐Liss, Inc.