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Carcino‐embryonic antigen may function as a chemo‐attractant in colorectal‐carcinoma cell lines
Author(s) -
Kim Jin C.,
Koo Kum H.,
Kim Byung S.,
Park Kun C.,
Bicknell David C.,
Bodmer Walter F.
Publication year - 1999
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19990909)82:6<880::aid-ijc18>3.0.co;2-s
Subject(s) - laminin , cell culture , carcinoma , colorectal cancer , cell , pathology , antigen , biology , carcinoembryonic antigen , cell adhesion , cell adhesion molecule , microbiology and biotechnology , cancer research , cancer , immunology , medicine , genetics
Locomotion of colorectal‐carcinoma cells was tested in order to establish whether it might be affected by carcino‐embryonic antigen (CEA). CEA production, cell growth and DNA ploidy were measured in 22 colorectal‐carcinoma cell lines. A cell‐invasion assay was adapted using a transfilter chamber, the lower surface of which was coated with various substrates in the amount of 5 μg/filter (CEA, type‐IV collagen, laminin). Cells infiltrated into the lower surface of the filter were counted over 9‐microscope fields (×400). All cell lines produced CEA, 9 producing more than 100 ng/ml medium. Of the total, 8 cell lines were diploid and 14 were aneuploid. Invasiveness, measured by the number of infiltrated cells, was highest in CEA‐coated filters, and next highest in type‐IV‐collagen‐ and laminin‐coated filters, in descending order ( p < 0.001–0.05). Invasiveness of each cell line was closely correlated with 2 substrates. Poorly differentiated or advanced‐stage tumors were more invasive than well‐differentiated or early‐stage tumors ( p < 0.001–0.05). However, invasiveness was not associated with DNA ploidy or CEA production. CEA may function as a chemo‐attractant as well as an adhesion molecule in colorectal‐carcinoma cell lines. In addition, adhesion to CEA appears to be related to type‐IV collagen and laminin. Int. J. Cancer 82:880–885, 1999. © 1999 Wiley‐Liss, Inc.