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Mother‐to‐child transmission of human T‐cell‐leukemia/lymphoma virus type I: Implication of high antiviral antibody titer and high proviral load in carrier mothers
Author(s) -
UretaVidal Abel,
AngelinDuclos Cristina,
Tortevoye Patricia,
Murphy Edward,
Lepère JeanFrançois,
Buigues RenéPierre,
Jolly Nicole,
Joubert Michel,
Carles Gabriel,
Pouliquen JeanFrançois,
Thé Guy de,
Moreau JeanPaul,
Gessain Antoine
Publication year - 1999
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19990909)82:6<832::aid-ijc11>3.0.co;2-p
Subject(s) - virology , provirus , tropical spastic paraparesis , serology , human t lymphotropic virus 1 , titer , antibody , virus , leukemia , immunology , polymerase chain reaction , population , medicine , biology , transmission (telecommunications) , peripheral blood mononuclear cell , human t lymphotropic virus , t cell leukemia , gene , genetics , electrical engineering , environmental health , engineering , genome , myelopathy , psychiatry , spinal cord , in vitro
In order to gain new insights into the risk factors influencing human‐T‐cell‐leukemia/lymphoma‐virus‐type‐I (HTLV‐I) mother‐to‐child transmission, a retrospective study of HTLV‐I infection among children born to HTLV‐I‐seropositive women was carried out in a highly HTLV‐I‐endemic population of African origin living in French Guyana. The study covered 81 HTLV‐I‐seropositive mothers and their 216 children aged between 18 months old and 12 years old. All plasma samples were tested for the presence of HTLV‐I antibodies by ELISA, immunofluorescence assay and Western blot. HTLV‐I provirus was detected, in the DNA extracted from peripheral‐blood mononuclear cells, by polymerase chain reaction (PCR) using primers specific for 3 different HTLV‐I genomic regions (LTR, gag and pX ) and quantified by a competitive PCR assay. Out of the 216 children, 21 were found to be HTLV‐I‐seropositive, giving a crude HTLV‐I transmission rate of 9.7%, while among the 180 breast‐fed children 10.6% were HTLV‐I‐seropositive. Perfect concordance between serological and PCR results was observed, and none of the 195 HTLV‐I‐negative children was found HTLV‐I‐positive by PCR. In conditional (by family) logistic‐regression models, HTLV‐I seropositivity in children was associated with an elevated maternal anti‐HTLV‐I‐antibody titer (OR 2.2, p = 0.0013), a high maternal HTLV‐I proviral load (OR 2.6, p = 0.033) and child's gender, girls being more frequently HTLV‐I‐infected than boys: OR 3.6, p = 0.0077 in the model including maternal anti‐HTLV‐I‐antibody titer and OR 4.1, p = 0.002 in the model including the maternal HTLV‐I proviral load. Int. J. Cancer 82:832–836, 1999. © 1999 Wiley‐Liss, Inc.