Premium
HLA‐A alleles of patients with pyothorax‐associated lymphoma: Anti‐Epstein‐Barr virus (EBV) host immune responses during the development of EBV latent antigen‐positive lymphomas
Author(s) -
Kanno Hiroyuki,
Ohsawa Masahiko,
Hashimoto Michiko,
Iuchi Keiji,
Nakajima Yutsuki,
Aozasa Katsuyuki
Publication year - 1999
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19990827)82:5<630::aid-ijc2>3.0.co;2-d
Subject(s) - biology , human leukocyte antigen , antigen , immunology , epstein–barr virus , immune system , virology , ctl* , virus , cytotoxic t cell , lymphoma , antibody , cd8 , genetics , in vitro
Pyothorax‐associated lymphoma (PAL) is an Epstein‐Barr virus (EBV) latent antigen‐positive lymphoma resembling EBV‐transformed lymphoblastoid cell lines (LCLs) and develops in non‐immunocompromised patients. Thus, deficient anti‐viral‐antigen immune responses might be involved in the development of PAL. As MHC class I–restricted cytotoxic T lymphocytes (CTLs) are the major constituent of anti‐viral immune responses, the HLA allele type and its expression may affect the development of PAL. Flow‐cytometric analyses of PAL cell lines and LCLs using the W6/32 monoclonal antibody revealed that expression of HLA class I varied among cell lines. Although one PAL cell line, OPL‐2, exhibited low expression, an LCL and another PAL cell line, OPL‐1, strongly expressed HLA class I. Among the EBV latent infection genes, EBV nuclear antigens 2, 3, 4 and 6 and latent membrane proteins can induce efficient CTL responses in combination with HLA‐A2 or ‐A11. HLA‐A alleles of PAL patients were determined using low‐resolution PCR‐based typing with HLA‐A locus sequence‐specific primer combinations. The antigen frequencies of HLA‐A2 and ‐A11 in PAL patients were not significantly different from those in the normal Japanese population. Although HLA class I antigen should be expressed during the course of lymphomagenesis, no HLA‐A alleles influenced the development of overt PALs. Int. J. Cancer 82:630–634, 1999. © 1999 Wiley‐Liss, Inc.