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Expression of the HMGI(Y) gene in human colorectal cancer
Author(s) -
Kim, DuckHwan,
Park YoungSuk,
Park Chul J.,
Son Kyu C.,
Nam Eun S.,
Shin Hyung S.,
Ryu JinWoo,
Kim Dae S.,
Park Cheol K.,
Park Young E.
Publication year - 1999
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19990820)84:4<376::aid-ijc8>3.0.co;2-n
Subject(s) - colorectal cancer , immunohistochemistry , carcinogenesis , biology , pathology , metastasis , reverse transcription polymerase chain reaction , cancer , lymph node , gene expression , cancer research , microbiology and biotechnology , medicine , gene , genetics
Expression of HMGI(Y), a nucleoprotein that binds to A/T rich sequences in the minor groove of the DNA helix, is observable in neoplastically transformed cells but not in normal cells. We have analyzed HMGI(Y) expression in colorectal cancer and evaluated its clinicopathologic significance. HMGI(Y) mRNA was measured by CRT‐PCR (competitive reverse transcription‐polymerase chain reaction). Immunohistochemical staining for HMGI(Y), p53 and Ki‐67 was performed in the same colon cancer tissues, and the results in colorectal tissues were similar to those of RT‐PCR. HMGI(Y) expression evidenced by RT‐PCR was observed in 63 of 64 (98.4%) colorectal cancer samples, and 2 of 5 (40%) adenomatous polyps, whereas 21 normal colon samples were negative ( p <0.001). High HMGI(Y) expression using CRT‐PCR was found in colon cancers with a high Ki‐67 labeling index ( p <0.001). There was no significant correlation between the levels of HMGI(Y) expression and stage, tumor size, lymph node metastasis, histologic grade and immunohistochemical status of p53. Our results indicate that the HMGI(Y) expression may occur at an early stage of carcinogenesis and correlate with cell proliferation. Int. J. Cancer (Pred. Oncol.), 84:376–380, 1999. © 1999 Wiley‐Liss, Inc.