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Rapid screening of specific changes in mRNA in thyroid carcinomas by sequence specific‐differential display: Decreased expression of acid ceramidase mRNA in malignant and benign thyroid tumors
Author(s) -
Maeda Ikuhiro,
Takano Toru,
Matsuzuka Fumio,
Maruyama Tokuyoshi,
Higashiyama Takuya,
Liu Gang,
Kuma Kanji,
Amino Nobuyuki
Publication year - 1999
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19990531)81:5<700::aid-ijc5>3.0.co;2-d
Subject(s) - thyroid , carcinogenesis , real time polymerase chain reaction , messenger rna , reverse transcription polymerase chain reaction , biology , adenoma , gene expression , pathology , thyroid carcinoma , cancer research , follicular phase , medicine , gene , endocrinology , cancer , biochemistry
Sequence specific‐differential display (SS‐DD) is a powerful method for screening significant changes in gene expression between normal and malignant tissues. Using this method, we detected 3 genes for which the expression is much decreased in thyroid tumors. After sub‐cloning and sequencing analysis, one of the genes was revealed to be acid ceramidase (AC). The expression of AC in normal thyroids and thyroid tumors was examined by semi‐quantitative reverse‐transcription‐polymerase‐chain‐reaction (RT‐PCR). Obvious decreases in the expression of AC mRNA were observed in 5/6 follicular adenomas, 2/2 adenomatous goiters, 3/6 papillary carcinomas and 1/2 follicular carcinomas. To confirm this result, real‐time quantitative PCR analysis (TaqMan PCR) was carried out. The relative expression level of AC mRNA compared with that of GAPDH mRNA was reduced in follicular adenomas, follicular carcinomas, and papillary carcinomas. Further, the expression of AC mRNA was extremely reduced in 2 anaplastic carcinomas. These results suggest a possible relationship between thyroid tumorigenesis and the expression of AC mRNA. Moreover, the increased expression of AC mRNA in normal thyroid tissues suggests some fundamental roles of AC in thyroid function. Int. J. Cancer 81:700–704, 1999. © 1999 Wiley‐Liss, Inc.