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Cyclin D1/PRAD1/BCL‐1 alternative transcript [B] protein product in B‐lymphoid malignancies with t(11;14)(q13;q32) translocation
Author(s) -
Hosokawa Yoshitaka,
Joh Tatsuroh,
Maeda Yumiko,
Arnold Andrew,
Seto Masao
Publication year - 1999
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19990517)81:4<616::aid-ijc18>3.0.co;2-s
Subject(s) - cyclin d1 , biology , chromosomal translocation , cyclin d , microbiology and biotechnology , oncogene , cell cycle , northern blot , cyclin b , b cell , cyclin , cancer research , cyclin a2 , messenger rna , gene , antibody , genetics
The cyclin‐D1/PRAD1 oncogene, a key regulator of the G 1 ‐phase progression of the cell cycle, has been identified as the long‐sought BCL‐1 oncogene in B‐cell malignancies with t(11;14)(q13;q32) translocation. A novel alternative spliced cyclin‐D1 transcript, called transcript[b], has been identified. The level of the variant transcript[b] was lower than that of the originally reported cyclin‐D1 transcript, called transcript[a], in several human non‐lymphoid cancer cell lines but the endogenous cellular expression of transcript[b] products has not yet been determined. Northern‐blot analysis and reverse‐transcription‐polymerase‐chain‐reaction(RT‐PCR) analysis revealed that transcript[b] mRNA is well expressed in B‐lymphoid cell lines with t(11;14)(q13;q32) translocation and at much lower or undetectable levels in other cells. Western‐blot analysis using a human cyclin‐D1‐specific monoclonal antibody, which can recognize and distinguish the products of transcripts [a] and [b], strongly suggested that the transcript [b] protein is indeed expressed in these B‐cell lines. The present study provides identification of the endogenous cellular expression of the cyclin‐D1‐transcript[b] protein and strongly suggests that this alternative form of cyclin D1 may play a significant role in the molecular pathogenesis of B‐lymphoid malignancies with t(11;14)(q13;q32) translocation. Int. J. Cancer 81:616–619, 1999. © 1999 Wiley‐Liss, Inc.