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Expression of the E2F family in human gastrointestinal carcinomas
Author(s) -
Suzuki Tetsuo,
Yasui Wataru,
Yokozaki Hiroshi,
Naka Kazuhito,
Ishikawa Takenori,
Tahara Eiichi
Publication year - 1999
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19990517)81:4<535::aid-ijc5>3.0.co;2-4
Subject(s) - medicine , cancer research , pathology , biology , oncology
The E2F family of transcription factors plays a key role in the control of cell‐cycle progression. Some family members may act as oncogenes, others as tumor‐suppressor genes. The genetic changes and the expression of E2F‐1 and ‐3 were examined in human gastric and colorectal carcinomas by Southern, Northern and Western blots. Gene amplification of E2F‐1 was detected in 4% (1/23) of gastric carcinomas and 25% (3/12) of the colorectal carcinomas. Increased expression of E2F‐1 mRNA was observed in 40% (12/30) of the gastric carcinomas and in 60% (3/5) of the colorectal carcinomas in comparison with the corresponding non‐neoplastic mucosa. Over‐expression of E2F‐1 protein was confirmed in many of the gastric carcinomas. In contrast, expression levels of E2F‐3 mRNA were lower in 70% (21/30) of the gastric carcinomas and in 20% (1/5) of the colorectal carcinomas than in their corresponding normal conterparts, while gross alteration of the E2F‐3 gene was not detected. These results suggest that gene amplification and anomalous expression of the E2F gene may permit development of gastrointestinal carcinomas. Int. J. Cancer 81:535–538, 1999. © 1999 Wiley‐Liss, Inc.