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Establishment of a human hemangiosarcoma cell line (ISO‐HAS)
Author(s) -
Masuzawa Mikio,
Fujimura Takao,
Hamada Yuhko,
Fujita Yuhsuke,
Hara Hisamichi,
Nishiyama Shigeo,
Katsuoka Kensei,
Tamauchi Hidekazu,
Sakurai Yoshinori
Publication year - 1999
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19990412)81:2<305::aid-ijc22>3.0.co;2-z
Subject(s) - cell culture , microbiology and biotechnology , hemangiosarcoma , cd31 , biology , endothelial stem cell , angiogenesis , cancer research , angiosarcoma , pathology , medicine , biochemistry , in vitro , genetics
A cell line (ISO‐HAS) has been established from tumor tissue of a human hemangiosarcoma arising on the scalp by the use of conditioned medium from a murine‐phenotypic angiosarcoma cell line (ISOS‐1). Cells have been cultured for more than 2 years with up to 100 passages. The cells retained endothelial‐cell properties, such as a characteristic cobblestone appearance at confluency, contact‐inhibited growth, active uptake of acetylated low‐density lipoprotein labeled with 1,1‐dioctadecyl 1,3,3,3,3‐tetramethyl‐indocarbocyanine perchlorate (Dil‐Ac‐LDL) and CD31 expression. However, they were weakly positive for von‐Willebrand‐factor (vWf) antigen and for binding of Ulex europaeus agglutinin‐I (UEA‐1) lectin, and lacked tube‐formation activity. These findings indicate that ISO‐HAS is a poorly differentiated endothelial cell line. ISO‐HAS cells showed accumulation of p53 protein in the nuclei, and a new‐typed p53 ‐gene point mutation was found in exon 7 at codon 240. When inoculated s.c. into severe‐combined‐immunodeficiency (SCID) mice, the cells showed solid‐tumor growth that caused death. These properties suggest that ISO‐HAS is a malignant endothelial cell line with high tumorigenicity. Int. J. Cancer 81: 305–308, 1999. © 1999 Wiley‐Liss, Inc.