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EB/RP gene family encodes tubulin binding proteins
Author(s) -
Juwana JanPeter,
Henderikx Paula,
Mischo Axel,
Wadle Andreas,
Fadle Natalie,
Gerlach Klaus,
Arends Jan Willem,
Hoogenboom Hennie,
Pfreundschuh Michael,
Renner Christoph
Publication year - 1999
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19990412)81:2<275::aid-ijc18>3.0.co;2-z
Subject(s) - gene , genetics , tubulin , biology , gene family , microbiology and biotechnology , microtubule , gene expression
Mutations in the adenomatous polyposis coli ( APC ) gene are linked to the dysplastic transformation of colorectal polyps and represent an early step in the development of colorectal tumors. Ninety‐four percent of all mutations result in the expression of a truncated APC protein lacking the C‐terminal region. The C‐terminal region of the APC protein may have a tumor suppressor function as its absence appears to be linked to the development of dysplastic lesions. Recently, we discovered and characterized a protein called RP1 which binds specifically to the C‐terminal region of the APC protein. We show now that RP1 and the other known members of the EB/RP family (EB1 and RP3) also bind directly to tubulin, both in vitro and in vivo. Immunohistochemical analyses reveal a distinct staining pattern during interphase as well as an association of RP1/EB1 with mitotic microtubule structures. The previously described puncta of the APC protein at the leading edge of membrane protrusions contact microtubule fibers that contain RP1 or EB1. Int. J. Cancer 81:275–284, 1999. © 1999 Wiley‐Liss, Inc.