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Detailed marker chromosome analysis in cell line U‐BLC1, established from transitional‐cell carcinoma of the bladder
Author(s) -
Bruch Jochen,
Wöhr Gudrun,
Brüderlein Silke,
Barbi Gotthold,
Wolter Hubertus,
Dixkens Christa,
Mattfeldt Torsten,
Möller Peter,
Paiss Thomas,
Hautmann Richard,
Vogel Walther,
Hameister Horst
Publication year - 1999
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19990315)80:6<903::aid-ijc17>3.0.co;2-8
Subject(s) - microdissection , karyotype , comparative genomic hybridization , transitional cell carcinoma , biology , metaphase , microbiology and biotechnology , locus (genetics) , urinary bladder , laser capture microdissection , chromosome , marker chromosome , carcinoma , pathology , gene , genetics , bladder cancer , medicine , gene expression , cancer
A permanent cell line, U‐BLC1, was established from a primary transitional‐cell carcinoma, TCC, of the urinary bladder. Karyotype analysis showed the line to be highly aberrant, with a near‐triploid chromosome number of 68 to 73. Comparative genomic hybridization revealed some distinct differences between the primary tumor and the established cell line. Karyotype analysis showed 3 marker chromosomes with homogeneously staining regions, HSRs, in the cell line. The HSRs were isolated by microdissection and the microdissection probes were hybridized to normal metaphase chromosomes. The HSRs contain sequences known to be frequently involved in amplification in transitional‐cell carcinoma of the bladder, 6p22, 7p11‐p12, 9p23‐pter, and one region not yet reported to be amplified in primary TCC of the bladder, 1p31‐p32. A candidate‐gene approach showed that in the region 7p11‐p12 the EGFR locus is amplified and highly expressed. Int. J. Cancer 80:903–910, 1999. © 1999 Wiley‐Liss, Inc.