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Human gamma delta T cells recognize heat shock protein‐60 on oral tumor cells
Author(s) -
Laad Anupama D.,
Thomas M. Loui,
Fakih Abdul R.,
Chiplunkar Shubhada V.
Publication year - 1999
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19990301)80:5<709::aid-ijc14>3.0.co;2-r
Subject(s) - cytotoxic t cell , flow cytometry , cytotoxicity , microbiology and biotechnology , monoclonal antibody , biology , cancer research , immunology , antibody , medicine , in vitro , biochemistry
In the present investigations, γδ T cells were isolated from the peripheral blood of oral cancer patients and analyzed for their immunophenotype and cytotoxic potential. Flow‐cytometric analysis revealed a dominant population expressing Vγ9 and Vδ2 T‐cell receptors. In a 4‐hr 51 Cr‐release assay, activated γδ T cells showed specific cytotoxicity against Daudi Burkitt's lymphoma cells and fresh oral tumor cells. Cold target competition assays demonstrated that γδ T cells recognize a common ligand on Daudi and oral tumor cells. Expression of heat shock protein 60 (hsp60) molecules was detected on the surface of Daudi as well as oral tumor cells by flow cytometry and immunoprecipitation of surface biotinylated cells by anti‐hsp60 monoclonal antibody (MAb). Such MAbs brought about a significant inhibition of cytotoxicity of γδ T cells against Daudi and oral tumor cells. The results suggest that γδ T cells isolated from the peripheral blood of oral cancer patients have the ability to lyse oral tumor cells. The lysis of oral tumor cells occurs via recognition of hsp60 on the surface of oral tumor cells. Int. J. Cancer 80:709–714, 1999. © 1999 Wiley‐Liss, Inc.