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Molecular characteristics of poorly differentiated adenocarcinoma and signet‐ring‐cell carcinoma of colorectum
Author(s) -
Kawabata Yuichi,
Tomita Naohiro,
Monden Takushi,
Ohue Masayuki,
Ohnishi Tadashi,
Sasaki Masaya,
Sekimoto Mitsugu,
Sakita Isao,
Tamaki Yasuhiro,
Takahashi Juro,
Yagyu Toshio,
Mishima Hideyuki,
Kikkawa Nobuteru,
Monden Morito
Publication year - 1999
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19990219)84:1<33::aid-ijc7>3.0.co;2-z
Subject(s) - signet ring cell carcinoma , adenocarcinoma , signet ring cell , carcinoma , pathology , medicine , oncology , cancer research , cancer
In a series of 45 poorly differentiated adenocarcinomas (por) and 7 signet‐ring‐cell carcinomas (sig) of the colorectum, K‐ ras gene mutation, p53 immunostaining and microsatellite instability (MSI) were analyzed for a comparison with 46 cases of colorectal carcinomas of the well or moderately differentiated type (well/mod). In addition, the mutations of simple repeated sequences in the transforming‐growth‐factor‐β type‐II receptor ( T β R‐II ) gene and the BAX gene were analyzed as possible targets for DNA replication errors. Mutation of the K‐ ras gene in the por, sig and well/mod specimens was detected in, respectively, 22%, 11% and 48%, positive immunostaining for p53 in 41.8%, 28.6% and 60.3%, and MSI in 36%, 30% and 4%. Frameshift mutation of the T β R‐II gene was detected in 27.5% of the por and none of the sig specimens, while corresponding figures for mutation of the BAX gene were 15.7% and 0%. Significant differences between the por and well/mod tumors were found in the occurrence of K‐ ras mutation at codons 12 and 13, and MSI. Clinicopathologically, the tumor status of por with MSI was found to significantly correlate with the tumor's location in the proximal colon. In cases without MSI and sig, no frameshift mutation of either the T β R‐II or the BAX gene was found. These results suggest that poorly differentiated and signet‐ring‐cell carcinomas have a genetic background different from that of well or moderately differentiated carcinomas of the colorectum, and that DNA‐replication error is at least partly involved in the carcinogenesis of these specific types of colorectal carcinomas. Int. J. Cancer (Pred. Oncol.) 84:33–38, 1999. © 1999 Wiley‐Liss, Inc.