Premium
Reduction of syndecan‐1 mRNA in cervical‐carcinoma cells is involved with the 3′ untranslated region
Author(s) -
Nakanishi Kazuyoshi,
Yoshioka Naohisa,
Oka Kiyomasa,
Hakura Akira
Publication year - 1999
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19990209)80:4<527::aid-ijc8>3.0.co;2-y
Subject(s) - syndecan 1 , untranslated region , hacat , biology , microbiology and biotechnology , transfection , messenger rna , keratinocyte , cell culture , cancer research , complementary dna , cell , gene , genetics
Syndecan‐1 is a transmembrane proteoglycan expressed predominantly in epithelial cells. Studies with immunohistochemistry have shown that syndecan‐1 expression is reduced in carcinoma derived from human epidermis. Here we show that syndecan‐1 mRNA, which is abundant in human primary keratinocyte (HK) and HaCaT spontaneous immortalized keratinocyte, is decreased in cervical‐carcinoma cell lines. Further, in relation to a long and well‐conserved 3′ untranslated region (3′ UTR) of syndecan‐1 cDNA, we examined whether 3′ UTR is involved with syndecan‐1‐mRNA reduction in cervical‐carcinoma cells. A stable transfection experiment showed that addition of the 3′ UTR does not affect expression in HaCaT, but that syndecan‐1 cDNA containing the 3′ UTR is not expressed efficiently selectively in cervical‐carcinoma cell lines. The transient assay with CAT reporter plasmids linking the 3′ UTR confirmed this, and indicated that the 3′ end of the 3′ UTR (nt 2285–2410) is required to influence expression in cervical‐carcinoma cells. Further excessive expression of syndecan‐1 suppressed growth in cervical‐carcinoma cells. These results demonstrate that the reduction of syndecan‐1 mRNA involved with the 3′ untranslated region gives growth advantage to cervical‐carcinoma cells. Int. J. Cancer 80:527–532, 1999. © 1999 Wiley‐Liss, Inc.