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Characterization of paired tumor and non‐tumor cell lines established from patients with breast cancer
Author(s) -
Gazdar Adi F.,
Kurvari Venkatesh,
Virmani Arvind,
Gollahon Lauren,
Sakaguchi Masahiro,
Westerfield Max,
Kodagoda Duli,
Stasny Victor,
Cunningham H. Thomas,
Wistuba Ignacio I.,
Tomlinson Gail,
Tonk Vijay,
Ashfaq Raheela,
Leitch A. Marilyn,
Minna John D.,
Shay Jerry W.
Publication year - 1998
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19981209)78:6<766::aid-ijc15>3.0.co;2-l
Subject(s) - breast cancer , primary tumor , cancer research , cell culture , medicine , estrogen receptor , pathology , cancer , metastasis , biology , oncology , genetics
The goal of our study was to develop a panel of tumor cell lines along with paired non‐malignant cell lines or strains collected from breast cancers, predominantly primary tumors. From a total of 189 breast tumor samples consisting of 177 primary tumors and 12 metastatic tissues, we established 21 human breast tumor cell lines that included 18 cell lines derived from primary tumors and 3 derived from metastatic lesions. Cell lines included those from patients with germline BRCA1 and FHIT gene mutations and others with possible genetic predisposition. For 19 tumor cell lines, we also established one or more corresponding non‐malignant cell strains or B lymphoblastoid (BL) lines, which included 16 BL lines and 7 breast epithelial (2) or stromal (5) cell strains. The present report describes clinical, pathological and molecular information regarding the normal and tumor tissue sources along with relevant personal information and familial medical history. Analysis of the breast tumor cell lines indicated that most of the cell lines had the following features: they were derived from large tumors with or without axillary node metastases; were aneuploid and exhibited a moderate to poorly differentiated phenotype; were estrogen receptor (ER)‐ and progesterone receptor (PR)‐negative; and overexpressed p53 and HER2/neu proteins. Of 13 patients with primary breast cancers receiving curative intent mastectomies, 7 were dead after a mean period of 10 months. Our panel of paired tumor and non‐malignant cell lines should provide important new reagents for breast cancer research. Int. J. Cancer 78:766–774, 1998. © 1998 Wiley‐Liss, Inc.