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Microsatellite alterations and p53, TGFβRII, IGFIIR and BAX mutations in sporadic non‐small‐cell lung cancer
Author(s) -
Caligo Maria A.,
Ghimenti Chiara,
Marchetti Antonio,
Lonobile Antonino,
Buttitta Fiamma,
Pellegrini Silvia,
Bevilacqua Generoso
Publication year - 1998
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19981123)78:5<606::aid-ijc13>3.0.co;2-t
Subject(s) - frameshift mutation , microsatellite instability , biology , point mutation , microsatellite , single strand conformation polymorphism , microbiology and biotechnology , gene , cancer research , mutation , genetics , allele
Fifty‐two sporadic primary non‐small‐cell lung carcinomas (NSCLC) were examined for microsatellite instability. Six different microsatellite markers localized on chromosomes 2, 5, 8, 10, 11 and 17 were used. Genomic instability was observed in 35% (18/52) of NSCLC at single or multiple loci. The tumors were also analyzed for p53 ‐gene mutations by PCR‐SSCP analysis. Polynucleotide stretch frameshift mutations of TGF β RII (transforming‐growth‐factor‐beta receptor II), IGFIIR (insuline growth‐factor II receptor) and BAX genes were also analyzed. RER + (replication‐error‐positive) tumors appear not to be affected by a higher rate of point mutations in coding sequences: no correlation was found between microsatellite instability and point mutations in the p53 gene, and the RER + tumors showed no alterations in stretches of nucleotide inside TGF β RII, BAX or IGFIIR. Int. J. Cancer 78:606–609, 1998. © 1998 Wiley‐Liss, Inc.