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Allelic loss on chromosome 9 in bladder cancer tissues and urine samples detected by blunt‐end single‐strand DNA conformation polymorphism
Author(s) -
Shigyo Masanori,
Sugano Kokichi,
Fukayama Noriko,
Taniguchi Takahiro,
Tobisu Kenichi,
Fujimoto Hiroyuki,
Doi Naoto,
Niwakawa Masashi,
Shimamura Kayako,
Tsukamoto Taiji,
Sekiya Takao,
Kakizoe Tadao
Publication year - 1998
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19981109)78:4<425::aid-ijc6>3.0.co;2-w
Subject(s) - microbiology and biotechnology , dna , bladder cancer , urine , allele , polymorphism (computer science) , biology , single strand conformation polymorphism , genetics , pathology , cancer , gene , polymerase chain reaction , medicine , endocrinology
Abstract Allelic loss on chromosome 9 is the most frequent and earliest genetic event in bladder carcinogenesis, and its detection in urine samples would be useful for detecting bladder cancer. A highly sensitive method to detect loss of heterozygosity (LOH) at 5 polymorphic loci on chromosome 9p and 9q was developed by the use of blunt‐end single‐strand DNA conformation polymorphism (blunt‐end SSCP) analysis. Tumor tissues, urine samples and peripheral blood lymphocytes from 34 patients with transitional cell carcinoma of the bladder were analyzed. LOHs on 9p and/or 9q were found in 24 (71%) of 34 tumor samples and 23 (70%) of 33 urine samples, while no allelic loss was detected in 20 urine samples from benign urothelial diseases. The frequency of allelic loss in tumor tissues was 67%, 71% and 80% in the pTa, pT1 and ≥pT2 stages and 50%, 80% and 79% in G1, G2 and G3 tumors, respectively. In comparison with a urine cytological examination, LOH on chromosome 9 was detected in 70% of urine samples diagnosed as transitional cell carcinoma, 67% of those as atypia and 70% of those as no malignant cells. Thus, detection of LOH on chromosome 9 from urine samples by blunt‐end SSCP is a more sensitive diagnostic modality than cytologic examination for detecting bladder cancer. It would be useful for postoperative management of bladder cancer, particularly when the allelic loss is revealed in the tumor tissues obtained at first surgery. Int. J. Cancer 78:425–429, 1998. © 1998 Wiley‐Liss, Inc.