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Up‐regulation of human secreted frizzled homolog in apoptosis and its down‐regulation in breast tumors
Author(s) -
Zhou Zhongjun,
Wang Jianming,
Han Xiaoliang,
Zhou Jiannian,
Linder Stig
Publication year - 1998
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19980925)78:1<95::aid-ijc15>3.0.co;2-4
Subject(s) - wnt signaling pathway , frizzled , carcinogenesis , biology , apoptosis , cancer research , cell growth , cell cycle , cell culture , signal transduction , cancer , endocrinology , medicine , microbiology and biotechnology , genetics
In the screening of apoptosis‐related genes, an elevated 4.5‐kb transcript representing the full‐length cDNA of human secreted frizzled‐related protein (hsFRP) was cloned. To investigate its possible role in the regulation of cell proliferation, gene expression of hsFRP was examined in human immortalized breast epithelial cell line HBL‐100 during growth arrest and apoptosis. Serum deprivation caused G 1 arrest and induction of hsFRP. When serum was re‐introduced into the cell culture, the expression of hsFRP declined. Adriamycin treatment induced accumulation of hsFRP mRNA and decrease of β‐catenin. This indicates that the regulation of hsFRP may be involved in the cell‐cycle/apoptosis mechanism and possibly in the wnt signaling pathway. hsFRP transcripts were undetectable in cells derived from malignant breast carcinomas, but detectable in 3 immortalized non‐malignant breast epithelial cell lines, indicating the involvement of hsFRP in the breast malignant transformation. When tumor and adjacent normal tissues from the same patients were examined, lower expression was found in 5/5 of breast tumors, 2/4 of ovary tumors and 3/5 of kidney tumors. These data suggest the possible involvement of hsFRP in regulation of cell proliferation and breast tumorigenesis. Int. J. Cancer 78:95–99, 1998.© 1998 Wiley‐Liss, Inc.