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Several genes encoding ribosomal proteins are over‐expressed in prostate‐cancer cell lines: Confirmation of L7a and L37 over‐expression in prostate‐cancer tissue samples
Author(s) -
Vaarala Markku H.,
Porvari Katja S.,
Kyllönen Atte P.,
Mustonen Mika V. J.,
Lukkarinen Olavi,
Vihko Pirkko T.
Publication year - 1998
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19980925)78:1<27::aid-ijc6>3.0.co;2-z
Subject(s) - lncap , prostate cancer , biology , suppression subtractive hybridization , prostate , in situ hybridization , cancer , ribosomal protein , pca3 , northern blot , complementary dna , microbiology and biotechnology , messenger rna , cdna library , cancer research , gene , ribosome , rna , genetics
A cDNA library specific for mRNA over‐expressed in prostate cancer was generated by subtractive hybridization of transcripts originating from prostatic hyperplasia and cancer tissues. cDNA encoding ribosomal proteins L4, L5, L7a, L23a, L30, L37, S14 and S18 was found to be present among 100 analyzed clones. Levels of ribosomal mRNA were significantly higher at least in one of the prostate‐cancer cell lines, LNCaP, DU‐145 and PC‐3, than in hyperplastic tissue, as determined by slot‐blot hybridization. Furthermore, L23a‐ and S14‐transcript levels were significantly elevated in PC‐3 cells as compared with those in the normal prostate epithelial cell line PrEC. Generally, dramatic changes in the mRNA content of the ribosomal proteins were not detected, the most evident over‐expression being that of L37 mRNA, which was 3.4 times more abundant in LNCaP cells than in hyperplastic prostate tissue. The over‐expression of L7a and L37 mRNA was confirmed in prostate‐cancer tissue samples by in situ hybridization. Elevated cancer‐related expression of L4 and L30 has not been reported, but levels of the other ribosomal proteins are known to be increased in several types of cancers. These results therefore suggest that prostate cancer is comparable with other types of cancers, in that a larger pool of some ribosomal proteins is gained during the transformation process, by an unknown mechanism. Int. J. Cancer 78:27–32, 1998.© 1998 Wiley‐Liss, Inc.