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Delineation of the breakpoint at 18q21.1 in a cell line (KARPAS1106) derived from mediastinal B‐cell lymphoma by fluorescence in situ hybridization with multiple YAC clones
Author(s) -
Tamura Akiko,
Akagi Tomoaki,
Nakazawa Naozo,
Kashima Kei,
Nakamura Shigeo,
Karpas Abraham,
Silverman Gary A.,
Morishima Yasuo,
Taniwaki Masafumi,
Seto Masao
Publication year - 1998
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19980925)78:1<100::aid-ijc16>3.0.co;2-f
Subject(s) - breakpoint , lymphoma , in situ , in situ hybridization , biology , genetics , chemistry , immunology , chromosome , gene , gene expression , organic chemistry
The breakpoint of the 18q21 translocation of B‐cellnon‐Hodgkin's lymphoma (NHL) cell line Karpas1106P was delineated by fluorescence in situ hybridization (FISH). Karpas1106P was derived from mediastinal lymphoblastic B‐cell lymphoma and exhibited the immunophenotype characteristic of marginal‐zone B‐cell lymphoma (MZL): smIg + , pan‐B antigen + , CD5 − , CD10 − and CD23 − . The original G‐banded karyotype showed a complex translocation containing t(X;18;13)(q28;q21;q12.1). Double‐color FISH (DCFISH) with whole‐chromosome‐painting (WCP) probes for chromosomes X, 13 and 18, and 18q‐specific yeast artificial chromosome (YAC) clones defined t(X;18;13) as ider(X)t(X;18;13)(q28;q12.3q21.1;q12.1). The immunoglobulin‐heavy‐chain ( IgH ) gene was not involved in the chromosomal translocation as detected by DCFISH with VH and C γ probes. By using contiguous YAC clones mapped from 18q12.3 to q21.1, we identified a YAC clone y852H2 with its breakpoint at 18q21.1. In Karpas1106P, the distal part of chromosome 18 from the breakpoint (18q21.1‐qter) was deleted, showing loss of heterozygosity of this region. In addition, the chromosomal segment 18q21.1 was duplicated and inserted to ider(X)t(X;18;13) between Xq28 and 13q12.1 with maintaining its original orientation. The DNA sequence of the breakpoint region contained in y852H2 can serve as a candidate locus for further molecular dissection to identify the causative gene of MZL. Int. J. Cancer 78:100–105, 1998.© 1998 Wiley‐Liss, Inc.