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Immunohistochemical expression of BRCA2 protein and allelic loss at the BRCA2 locus in prostate cancer
Author(s) -
Edwards Stephen M.,
Dunsmuir William D.,
Gillett Cheryl E.,
Lakhani Sunil R.,
Corbishley Catherine,
Young Martin,
Kirby Roger S.,
Dearnaley David P.,
Dowe Anna,
ArdernJones Audrey,
Kelly Jo,
Spurr Nigel,
Barnes Diana M.,
Eeles Rosalind A.
Publication year - 1998
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19980925)78:1<1::aid-ijc1>3.0.co;2-u
Subject(s) - immunohistochemistry , prostate cancer , locus (genetics) , allele , protein expression , biology , prostate , brca2 protein , pathology , genetics , cancer research , medicine , cancer , gene , mutation , germline mutation
Many epidemiological studies have reported an association between breast and prostate cancer. BRCA2 functions as a tumour‐suppressor gene in about 35% of large familial breast‐cancer clusters; its role in the pathogenesis of sporadic breast cancer is less clear. We have evaluated immunohistochemical expression of BRCA2 protein and allelic loss of markers at the BRCA2 locus in tissue derived both from sporadic and from familial cases of prostate cancer. Immunohistochemical analysis was performed in 167 paraffin‐embedded archival specimens. Normal prostate and 75% (120/160) of prostate‐cancer tissue did not express BRCA2 protein. However, 25% (40/160) of cancer cases did express patchy staining; of these, 17% (27/160) expressed positive nuclear staining in normal glandular tissue adjacent to tumour (either in addition to, or, independent of tumour). Allelic loss is the hallmark of a tumour‐suppressor gene. Markers flanking (D13S267, D13S260) and within (D13S171) the BRCA2 gene indicated allelic loss in at least one locus in 23% (17/73) of tumours analyzed. There was no difference in the rates of allelic loss between sporadic and familial tumours, nor was there any association between immunohistochemical staining and allelic loss. Although immunohistochemical staining provided no useful prognostic information, allelic loss at BRCA2 was shown in univariate analysis to be associated with poorer survival (log‐rank test, p = 0.046). Int. J. Cancer 78:1–7, 1998.© 1998 Wiley‐Liss, Inc.