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Suppression of human fibrosarcoma cell growth by transcription factor, Egr‐1, involves down‐regulation of Bcl‐2
Author(s) -
Huang RuoPan,
Fan Yan,
Peng Ao,
Zeng ZiLi,
Reed John C.,
Adamson Eileen D.,
Boynton Alton L.
Publication year - 1998
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19980911)77:6<880::aid-ijc14>3.0.co;2-5
Subject(s) - fibrosarcoma , transcription factor , cancer research , cell growth , biology , microbiology and biotechnology , genetics , gene
Previously, we showed that the transcription factor Egr‐1 suppressed the proliferation of v‐ sis transformed NIH3T3 cells and also a number of human tumor cells. Here, we investigate the possible mechanisms responsible for this function. We show that transfected Egr‐1 in human fibrosarcoma cells HT1080 leads to down‐regulation of Bcl‐2. Transient CAT transfection assays reveal that expression of Egr‐1 suppresses Bcl‐2 promoter activity in a dose‐dependent manner. Furthermore, overexpression of Bcl‐2 in Egr‐1‐expressing HT1080 cells enhanced cell proliferation in monolayer culture and increased anchorage‐independent growth. Our results suggest that suppression of tumor cell proliferation by Egr‐1 may be at least partially mediated through the down‐regulation of Bcl‐2. Int. J. Cancer 77:880‐886, 1998.© 1998 Wiley‐Liss, Inc.