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Dominant expression of sex‐hormone‐binding‐globulin exon‐7 splicing variant over wild‐type mRNA in human ovarian cancers
Author(s) -
Misao Ryou,
Nakanishi Yoshihito,
Fujimoto Jiro,
Iwagaki Shigenori,
Tamaya Teruhiko
Publication year - 1998
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19980911)77:6<828::aid-ijc5>3.0.co;2-w
Subject(s) - exon , sex hormone binding globulin , biology , rna splicing , messenger rna , alternative splicing , endocrinology , medicine , gene , hormone , genetics , rna , androgen
The intracellular expression of sex‐hormone‐binding‐globulin(SHBG) exon‐7‐splicing‐variant mRNA in human ovarian cancers was demonstrated by reverse‐transcription/polymerase‐chain‐reaction, Southern‐blot and DNA‐sequencing analyses. Analysis of the missing base pairs proved that they corresponded to the entire exon 7, which is considered to encode a portion of the steroid‐binding site, suggesting that the steroid‐binding affinity of this variant might be different from that of the SHBG wild type. SHBG wild‐type and variant mRNA was detected in all normal ovaries and in benign and malignant ovarian tumors analyzed. There were no significant differences in mean SHBG wild‐type and variant mRNA levels among the 3 types of tissue, but the ratio of SHBG exon‐7‐splicing‐variant to wild‐type mRNA level in ovarian cancers was significantly higher ( p < 0.05) than that in normal ovaries, with over‐expression in some benign tumors. SHBG mRNA levels and the ratio of SHBG variant to wild‐type mRNA was not associated with histological classification or clinical stages of ovarian cancers. These results suggest that over‐expression of SHBG‐exon 7‐splicing‐variant mRNA to the wild type might indicate the potential for neoplastic transformation. Int. J. Cancer 77:828–832, 1998.© 1998 Wiley‐Liss, Inc.