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Changes in glutathione redox cycling and oxidative stress response in the malignant progression of NB2 lymphoma cells
Author(s) -
Meyer Timothy E.,
Liang Hanqian Q.,
Buckley Arthur R.,
Buckley Donna J.,
Gout Peter W.,
Green Edie H.,
Bode Ann M.
Publication year - 1998
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19980703)77:1<55::aid-ijc10>3.0.co;2-8
Subject(s) - glutathione , oxidative stress , biology , cancer research , lymphoma , medicine , endocrinology , biochemistry , immunology , enzyme
Differential analysis of closely related Nb2‐lymphoma cell lines can be used for identification of changes in biochemical properties associated with the malignant progression of certain T‐cell cancers. As tumors progress, they tend to show metabolic alterations such as an increased resistance to oxidative stress, a characteristic that may be correlated with changes in intrinsic antioxidant levels ( e.g., glutathione) and in activities of associated enzymes such as the glutathione redox pathway. Whether increases in malignancy of Nb2 cells were associated with changes in cellular glutathione levels and activities of glutathione‐metabolizing enzymes was addressed. To evaluate this relationship, 3 cell lines, showing increased malignancy, were used: Nb2‐U17 (hormone‐dependent, non‐metastatic), Nb2‐11 (hormone‐dependent, metastatic), Nb2‐SFJCD1 (growth factor‐independent, metastatic). Compared to Nb2‐U17 and Nb2‐11 cells, the highly progressed Nb2‐SFJCD1 lymphoma cells maintain low basal glutathione levels. However, the Nb2‐SFJCD1 cells display an enhanced capacity to produce glutathione when challenged with an oxidative stress and show a significantly higher resistance to H 2 O 2 ‐induced apoptosis. Int. J. Cancer 77:55–63, 1998.© 1998 Wiley‐Liss, Inc.